HPLC METHOD DEVELOPMENT FOR ESTIMATION GALLIC ACID AND ELLAGIC ACID IN AN AYURVEDIC ANTIARTHRITIC FORMULATION SIMHNAD GUGGUL
Keywords:Simhnad guggul, HPLC, Gallic acid, Ellagic acid.
Present study is a work on results of analytical method development for two important bioactive phyto-constituents of a well-known Ayurvedic formulation Simhnad guggul i.e. gallic acid and ellagic acid. TLC method was used for authentication of these constituents in formulation. Then HPLC methods were developed for quantification of gallic acid and ellagic acid in formulation. The optimized mobile phase for gallic acid was Acetonitrile: water (70:30) and 3% Glacial acetic acid and for ellagic acid it was Acetonitrile: water (80:20) and 1% Glacial acetic acid. The method was applied on market formulations and freshly prepared one. The results indicate that developed methods were found to be precise, sensitive, reliable and accurate.
2. Rasrasayankalp, (1966), ‘Simhnadguggul’, Bhaishajsamhita, Prakran 1, p – 517.
3. Ayurvedic Formulary of India,part I, p - 223-224.
4. S.Sangvikar, R. Singh, S. Pitta, R.Rana, (2017), ‘Clinical safety of selected Ayurvedic formulations in Rheumatoid Arthritis’, Journal of research in Ayurvedic Sciences, vol. 1(2), p- 108 to 116. DOI: 10.5005/jp-journals-10064-0011
5. H. K.Singhal, N. P.Mishra, A.Kataria, (2014), ‘A Comparative Study on Singhnadguggulu&Trayodasangaguggulu in the Management of Juvenile Rheumatoid Arthritis W.S.R to Amavata’, American Journal of Pharmacology and Pharmacotherapeutics, vol.1(3), p-76 to 81.
6. R. R. Mahto, A. R. Dave, V. D. Shukla, (2011), ‘A comparative study of RasonaRasnadiGhanavati and SimhanadaGuggulu on Amavata with special reference to Rheumatoid arthritis’, AYU, vol.32(1), p-46 to 54. DOI: 10.4103/0974-8520.85724
7. S. A.Pandey, N. P.Joshi, D. M.Pandya, (2012), ‘Clinical efficacy of Shiva Guggulu and SimhanadaGuggulu in Amavata (Rheumatoid Arthritis)’, Journal of research in Ayurveda, vol. 33(2), p-247 to 254.
8. V.N.Sumantran, A.A.Kulkarni, A.Harsulkar, A.Wele, S.J.Koppikar, R.Chandwaskar, V.Gaire, Dalvi M., U.V.Wagh, (2007), ‘Hyaluronidase and collagenase inhibitory activities of the herbal formulation Triphalaguggulu.’, Journal of Bioscience, vol.32(4), p – 755 to 61.
9. S. K.Debnath, S. N.Vyas, (2015), ‘Phytochemical Analysis of SimhanadGuggulu Pill’, International Ayurvedic Medical Journal, vol. 3(7), p-1 to3.
10. M.Rasool, E.P.Sabina, (2007), ‘Anti-inflammatory effect of the Indian Ayurvedic herbal formulation Triphala on adjuvant induced arthritis in mice’, Phytotherapy Research, Vol.21(9), p – 889 to 894.
11. S. S.Ganpathy, S. P., Kumar R., S.Kumar, C. D. J., L. R. Y., (2014), ‘In vivo, In vitro anti-arthritic studies of Ellagic acid from KirganeliareticulataBaill and its molecular docking’, Journal of Applied Pharmaceutical Science, vol. 4(7), p- 24 to 31.
12. G.Allam, E. A.Mahdi, A. M.Alzahrani, (2016), Abuelsaad A. S., ‘Ellagic acid alleviates adjuvant induced arthritis by modulation of pro- and antiinflammatory cytokines’, Central European Journal of Immunology, p-339 to 349.
13. L. A.Bensaad, K. H.Kim, C. C.Quah, W. R.Kim, M.Shahimi, (2017), ‘Anti-inflammatory potential of ellagic acid, allic acid and punicalagin A&B isolated from Punicagranatum’, BMC Complementary and Alternative Medicine, vol. 17(47), p-1 to 10. DOI 10.1186/s12906-017-1555-0
14. B. H.Kroes, A. J.VandenBerg, H. C.Quarles van Ufford, H.Van Dijk, R. P.Labadie, (2007), ‘Anti-inflammatory activity of Gallic acid’, PlantaMedica, doi. 10.1055/s-2006-961535.
15. S. K.Debnath, S. N.Vyas, (2009), ‘A clinical study on the management of amavata (rheumatoid arthritis) with simhanadaguggulu and alambushadichurna tablet.’ Ayurveda, Yoga & Naturopathy, Unani, Siddha, Homoeopathy and Sowa Rigpa, vol. 30(2), p – 164 to 170.